Why streaming? | Which chunk size to use? | Overview of the streaming API | Streaming using default callbacks | Assumptions | Obtaining the callbacks | Running the demultiplexing | Displaying the result from streaming | Streaming the barcode table | Considerations about size of tables | Subsetting the frequency table | Writing to database | Streaming using custom callbacks | Desired properties | The state object | The loader | The archiver | Putting it all together | Verifying results | Reproducibility | Bibliography

Basics | When and why to use this package | Required knowledge | Limitations | Ways to interact with this package | Install posDemux | Asking for help | Citing posDemux | An example with PETRI-seq | Quick overview of the method | Sequence annotation | Data loading | Running the demultiplexer | Error correction | Filtering by barcode frequency | Exporting results | Reproducibility | Bibliography

When and why to use this package | Installation | Some theoretical considerations | Workflow outline | How to we approach from here? | Considerations to note | Number of bootstrap iterations | Number of threads | Memory consumption | Number of top gene pairs to pick | Session info for this vignette | References